x

wild type

uploads/wild type.jpg

wild type 【生物學】野生型。W- West 〔或作 w- W-〕〔...

【生物學】野生型。W- West 〔或作 w- W-〕〔美國〕蠻荒的西部〔在早先拓荒時代的美國西部地區，該地當時無法律〕。 “wild“ 中文翻譯：   adj. 1.野生的 (opp. domestic; c ...“type“ 中文翻譯：   n. 1.型，類型，(工業產品的)品種；風格，型式。 2 ...“a type of wild soybean“ 中文翻譯：   一種野生大豆“a type of wild species“ 中文翻譯：   一種野生種“preexisting wild-type tumor“ 中文翻譯：   預成野生型腫瘤“virulent wild-type virus“ 中文翻譯：   毒性野型簿“wild type gene“ 中文翻譯：   野生型基因“wild type p53“ 中文翻譯：   野生型p53“wild type strain“ 中文翻譯：   野生株“wild type virus“ 中文翻譯：   野毒“wild-type strain“ 中文翻譯：   野生型菌株“be wild with“ 中文翻譯：   因…而欣喜例如“be-wild“ 中文翻譯：   野大司“in the wild“ 中文翻譯：   處于野生狀態; 在自然環境下“into the wild“ 中文翻譯：   阿拉斯加之死; 荒野生存; 走進野地“the wild“ 中文翻譯：   荒野歷險; 狂野大自然; 獸猛猛奇兵; 野蠻任務“wild“ 中文翻譯：   adj. 1.野生的 (opp. domestic; cultivated); 野性的，未馴養的。 2.未耕作的，荒野的；沒人住的，無人煙的，完全自然的。 3.未開化的，野蠻的。 4.狂暴的，狂亂的；任性的。 5.猛烈的，波濤洶涌的，狂風暴雨的。 6.瘋狂(似)的，熱狂的；瘋狂地想…。 7.魯莽的，莽撞的；荒唐的；古怪的；不著邊際的。 8.散亂的，邋遢的。 9.【牌戲】百搭的。 a wild beast 野獸。 a wild bird 野鳥。 a wild boar 野豬。 a wild flower 野花。 a wild park 天然公園。 wild fancies 狂想，妄想。 wild fluctuations (行市的)狂漲暴跌。 wild living 放蕩的生活。 He settled down after a wild youth. 他少年時候放蕩后來老實了。 a wild night 暴風雨之夜。 a wild sea 波濤洶涌的大海。 wild with joy 狂喜。 wild rage 暴怒。 a wild blow 亂打。 a wild pitch 亂投。 a wild shot 亂射。 wild times 亂世。 be wild about 1. 熱中于，給…迷住。 2. 因某事狂怒。 be wild with excitement 興奮若狂。 drive sb. wild 激怒某人。 go wild 興奮；高興。 grow wild 野生，自然生長。 make sb. wild 使某人生氣。 run wild 變野蠻；變粗暴；放肆起來；(植物)亂長。 wild and woolly 〔美國〕粗野的；莽撞的；(美國)西部的。 wild cats 〔美俚〕水兵。 wild willie west 〔美國〕供人參觀的西部牧場。 wild work 粗暴的動作。 adv. 狂暴地；猛烈地；胡亂地。 shoot wild 亂射。 talk wild 瞎講。 n. 〔常 pl.〕荒地，荒野；未開化的地方。 “the wild, wild rose“ 中文翻譯：   野玫瑰之戀“wild mountains；in the wild mountains“ 中文翻譯：   野山“wild nights wild nights“ 中文翻譯：   暴風雨夜“wild wild west“ 中文翻譯：   超智特務顯神通; 狂野西部; 飆風戰警; 飚風戰警“a wild elephant“ 中文翻譯：   野象“a wild fancy“ 中文翻譯：   不著邊際的空想“a wild fellow“ 中文翻譯：   放肆的家伙“a wild guess“ 中文翻譯：   不著邊際的臆測; 胡亂猜測; 胡亂猜想

wild west show

In this work , the transgenic plants showed a tendency to accumulate less na + and more k + under saline condition than wild type plants and there was a high cytosolic k + / na + ratio in the roots of transgenic lines . 5 . x - ray microanalysis of na + , k + contents of the symplast and apoplast showed that transgenic lines accumulate less na + and more k + in the symplast than wild type plants 在對轉基因植株及野生型植株用100nunol lnaci處理六天后， na ” 、 k ”的x一射線微區分析，轉基因植株根、葉中質外體na 、 k十含量變化不大，但共質體中na十含量明顯降低，廣含量高于對照。

Wild arabidopsis thaliana seeds were mutagenized with y - ray , and m2 populations were screened in the medium containing 200mmol / l nacl . 352 potential high - salt - tolerance mutants were selected from 80 , 000 m2 seedlings , and 4 of this m2 mutants showed high - salt - tolerance in their m3 generation . this high - salt - tolerance mutants were backcrossed with the wild type arabidopsis thaliana 在含200mmol lnacl的改良ms培養基上，通過對約80 , 000株m _ 2代幼苗的篩選，共獲得352株根仍表現向地性生長或真葉不變為褐色的植株作為可能的突變體。

This unusual modification causes wild type s . lividans 1326 dna sensitive to site - specific oxidative double - strand cleavage ( dnd phenotype ) . preliminary study revealed that such dna modification involves incorporation of sulphur . the entire functional dnd cluster , 8 . 3kb in size , including 3 orfs - dnda , dndb and dndc was involved in this dna modification 野生型變鉛青鏈霉菌具有一種不同于甲基化修飾的新型dna硫修飾系統，使dna在電泳時易遭到氧化雙鏈切割而導致降解( dnd , dnadegradation )表型( zhouetal . , 1988 , 1994 , 1999 ) 。

3 . northern analysis revealed the expression of sod2 mrna in t3 plants of several non - segregation transgenic lines while no signal was shown in wild type plants . several samples of transformants were self pollinated , and progenies from transformed and non - transformed plants ( controls ) were evaluated for salt tolerance Northern分析表明轉基因植株均有雜交信號，野生型植株無明顯信號，進一步說明sod2基因整合到擬南芥的基因組后已正常轉錄表達，由信號的強弱來看，表達量有明顯的不同，可能與整合的外源基因的拷貝數相關。

To prepare the wild type mbl in prokaryotic system , a pair of primers was designed and synthesized , and was used to amplify mbl gene from the recombinant vector pgem - mbl that contans wild type mbl cdna . a recombinant prokaryotic expression vector , pet28 - mbl , was constructed by inserted the mbl gene into plasmid pet28 ( b ) , and after transfected it into ecoli bl21 ( de3 ) and induced with iptg , recombinant mbl protein was expressed successfully 本實驗另選用了原核表達質粒pet28 ( b ) ，根據已構建好的含有mbl野生型基因的t載體pgem - mbl ，設計一對引物， pcr擴增mbl基因，凝膠回收，雙酶切pcr產物和pet28 ( b )質粒， t4連接酶連接，轉化大腸桿菌dhsa ，氨芋選擇培養挑取克隆鑒定。

Nacl ( loomm ) markedly induced the increasing in fluorescence of dcf in guard cells of wild type , but not in ios5 , and the fluorescence intensity in guard cells of wild type was nearly 230 % as much as in ios5 after 15 minutes treatment ; nacl ( loomm ) and aba ( loum ) together induced rapid increasing in fluorescence in both wild type and ios5 外源100mmnacl處理時野生型保衛細胞內熒光顯著增強，而los5保衛細胞內熒光只有輕微的增強，處理15分鐘時，野生型保衛細胞內熒光強度為los5的2 . 17倍； 100mmnacl與10 maba共處理可誘導野生型及los5胞內熒光均迅速增強， 5分鐘時熒光已布滿整個細胞。

The results were as followed : pollen germination and tube growth were decreased in ga mutant , and increased in ga overexpression strains as compared to wild type , while pollen germination and tube growth were not affected in gp mutant 結果表明：體外實驗中gpa1突變體花粉的萌發率及花粉管生長速率均低于野生型花粉，而超表達g花粉的萌發率及花粉管生長速率則高于野生型及空載體對照； g突變體agb1 - 1與野生型花粉的萌發率及花粉管生長速率差異不大。

The results represented upon observation of stomatal movement showed that the density of stoma in the epidermal of transgenic tobaccos was much lower than that of wild type and the rate of opening and closing of stoma increased because of strong expression oi atdofl . 7 我們利用rt - pcr的方法，從擬南芥中克隆了與在氣孔中表達較多的stdof1基因的同源基因atdof1 . 7 ，構建了該基因的超表達載體，獲得了t1代轉基因煙草苗。

A novel dna modification discovered in streptomyces lividans is different from dna methylation . this unusual modification causes wild type s . lividans dna sensitive to site - specific oxidative double - strand cleavage ( dnd phenotype , dna degradation ) 變鉛青鏈霉菌的dna異常修飾系統是一種不同于甲基化修飾的新型修飾系統，它可使變鉛青鏈霉菌dna在電泳時易遭到氧化雙鏈切割( dnd表型， dnadegradation ) ( zhouetal . , 1988 ) 。

It was also found that the average number of germinated pollen grains on each stigma of ga mutant was less than that of wild type , while in ga overexpression strains the average number was more than that of control ( wild type and empty vector control ) 體內實驗中， gpa1突變體柱頭上實際萌發的花粉粒數少于野生型，超表達植株則多于野生型和空載體對照； agb1 - 1突變體柱頭上實際萌發的花粉粒數與對照比較差異不顯著。

In order to reveal the mechanisms of extreme radioresistance and dna repair in deinococcus radiodurans , we examined proteome changes in a wild type strain following y - irradiation using 2 - dimensional polyacrylamide gel electrophoresis and silver staining 為了研究耐輻射球菌極強的輻射抗性與dna修復機理，我們應用雙向電泳結合銀染的方法考察了野生型菌株kd8301在射線照射前后細胞體內總蛋白組的變化情況。

1 oomm nacl induced the open stomata of wild type close effectively , while the effects of nacl on stomatal aperture of ios5 were not significant ; 1 oomm naci and loum aba effectively induced stomatal closure of both wild type and ios5 100mmnacl可有效誘導野生型開放的氣孔關閉，而對los5氣孔開度的影響不很明顯： 100mmnacl與10 m的aba共處理時，野生型及los5氣孔開度均明顯減小，且幅度類似。

Whether the gene was transformed into the arabidopsis was confirmed by pcr - southern blotting , southern blotting and northern blotting . 1 . pcr - southern blotting showed that all kanamycin - tolerant plants had strong positive signals , and no signal was shown in wild type plants Pcr結果擴增出1 . 4kb的特異性條帶，表明sod2已整合進擬南芥中， pcr - southern進一步證實了pcr結果的正確性。

However , the receptor and translocation domains of the colicin ia can only recognize the inner and outer membrane receptors of the escherichia coli , therefore the wild type colicin ia can not be developed into antibiotics against other types of bacteria 然而由于其信號識別結構域只能識別大腸桿菌外膜和內膜上的特異受體，因此野生型大腸菌素難以拓展成為對抗它種細菌的抗菌素。

H2o2 generation in guard cells was examined by laser scanning confocal microscopy based on fluorescence probe h2dcfda . the fluorescence intensity in guard cells of wild type and ios5 was essentially the same before treatment 以h2o2熒光探針h2dcfda結合激光掃描共聚焦顯微技術直接檢測了保衛細胞內的h2o2產生情況。未加任何處理之前，野生型與los5保衛細胞內熒光強度幾乎相等。

After the treatment of different concentrations nacl ( 0 - 200mmol / l ) , the fresh weight of the transgenic lines was dramaticly higher than that of wild type plants , the dry weight had no markedly differences between the transgenic plants and wild plants 在用不同濃度的naci （ 0 200mm 。 l l ）處理后，測其干、鮮重，結果表明轉基因植株鮮重明顯高于對照，干重差異不顯著。

Secondly another vector phz1117 which for replacement of sc ( j11 . 17 / scd78 . 01 , was constructed . phz1117 was transformed into wild type strain m145 and a gene replacement strain named hxy3 was obtained 其次，構建了另一個用于置換scq11 . 17 scd78 . 01的置換載體phz1117 ，得到基因置換菌株hxy3并通過southern雜交驗證，與野生型m145相比，沒有觀察到hxy3有明顯的表型變化。

Methode competence is induced in the s . pn detached from clinic and creat the come duplicaion mutants by insertion - duplication . use the real - time rt - pcr to test the expressing level of vurilence between the mutants and the wild type 8的ctm中生長的菌作為陰性對照。分別在生長的不同時間點提取rna ，最后采用rea卜timert ? pcr來定量測定各種毒力因子mrna表達的水平。

In most wild type e . coli strains containing heterologous pha synthase genes , the amount of pha accumulation was almost undetectable while in e . coli km32b the amount of pha accumulation were clearly visible 在多種野生型大腸桿菌中，盡管使用了各種不同的表達載體，也很難獲得可供檢測的pha ；但應用大腸桿菌的fqdb缺失突變株卻可以獲得較多量的pha 。